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Recent Articles

Mycotoxic Effectsn of Seed-Borne Fungi on Seed Health of Black Gram



 Green gram, Black gram, Pigeon pea and chickpea are common pulses in diet rich in carbohydrates, proteins and minerals. Numerous fungi affect pulses adversely causing reduction in seed content and seed health. During present study, effects of metabolites of seed-borne fungi on seed health are evaluated. Total seventeen fungi recorded from all test pulses. Out of these seventeen seed-borne fungi, six, Aspergillus flavus, A. fumigatus, A. niger, Drechslera tetramera and Rhizopus stolonifer, found to be common and dominant on four test pulses. These common and dominant seed-borne fungi produced mycotoxins that affected adversely to the seed germination, shoot and root length of test pulse Black gram in variable quantity.

Growth and Nutritional Properties of Pleurotus Sajor-caju Cultivated on Sawdust of an Exotic and Indigenous Tree Species


A graph representing the rate at which the two substrates used support the growth of pleurotus Sajor-cajo

 Pleurotus Sajor-caju was cultivated on the sawdust of Ceiba pentandra and Gmelina arborea with the aim of comparing the nutritional values of the mushrooms cultivated. The sawdust substrates were inoculated with mushroom spawn and the analysis of the nutritional value was carried out using the AOAC 2005 method . The result showed that the pleurotus Sajor-caju grown on the sawdust substrates of Ceiba pentandra has 23.36% protein, 70.42% fat, 3.58% crude fibre, 9.12% nitrogen, 57.02% moisture content, 59.99% organic matter and 29.98% nitrogen. While the mushroom grown on Gmelina arborea has 23.43% protein, 65.06%fat, 2.73% crude fiber, 9.15% nitrogen, 67.76% moisture content, 65.84% organic matter and 24.13% nitrogen. The result indicated that there was no significant difference in the nutritional values of Pleurotus Sajor-caju grown on the sawdust from the selected species. Also, ceiba pentandra supports the growth of pleurotus Sajor-caju more than Gmelina arborea when the total number of days it took both substrates to ramified and emerge spores.

Efficient in vitro regeneration of biodiesel Plant jatropha curcas .L


Jatropha curcas grown under field condition

 In the present investigation, in vitro propagation of Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) medium supplemented with 20 μM N6-benzyl- adenine (BA) and 50 μ M adenine sulphate, in which cultures produced 8.2 ± 0.56 shoots per nodal explant with 3.0 cm length after 3-5 weeks. The rate of shoot multiplication was significantly enhanced after transfer to MS medium supplemented with 2.5 μ M 6-furfuryl amino purine (Kn), 0.5 μ M indole- 3-butyric acid (IBA) and 25 μ M adenine sulphate for 4 weeks. Internode explant segments of Jatropha curcas plants responded in vitro and formed callus tissue when cultured on Murashige-Skoog (MS) full strength nutrient medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D – 4 mg/L) and N6-Benzyl adenine (BA- 4 mg/L). The internode-derived callus tissues were found non-embryogenic and hence did not regenerate into shoot and root, respectively. The internode segments when cultured on MS (full strength) media supplemented with BA – 5 mg/L) were found to grow forming two to three buds. However, these shooting explants did not form roots upon hormonal regulation. On the contrary, endosperm tissue cultured on full strength MS media supplemented with 3 mg/L BA and 1 mg/L Indole-3-butyric acid (IBA) along with activated charcoal (100 mg/L) and ascorbic acid (50 mg/L) yielded simultaneous shooting and rooting response after four weeks of incubation.